Table 2. Summary of anti-EBOV GP IgG ELISA qualification results.

Parameter	Result
Negative Sample OD	95% Prediction Interval of 0.000–0.337
Limit of Detection	31.74 ELISA units/mL
Lower Limit of Quantitation	55.34 ELISA units/mL
Upper Limit of Quantitation	2,511.89 ELISA units/mL
Intermediate Precision	14.8% CV
Repeatability	8.2% CV
Dilutional Linearity (Accuracy)	Slope of -0.99 (90% CI of -1.03, -0.94)
rGP-Coated Plate Stability	7 days at 2–8°C
Serum Stability	21 days at 2–8°C 24 hours at room temperature 7 freeze/thaw cycles
rGP Stability	3 days at 2–8°C 8 freeze/thaw cycles (6 recommended)