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. 2019 Apr 18;14(4):e0215727. doi: 10.1371/journal.pone.0215727

Fig 2. Monitoring the presence of virus in cornea of infected mice.

Fig 2

Mice were ocularly infected with HSV-1 as described above and viral titers were measured in the eye of infected mice by plaque assay 12h, 1, 3, 5 and 7 days PI using eye swabs from 20 eyes. At the indicated time points, corneas of infected mice were isolated and single cell suspensions were prepared as described in Fig 1 above. Single cell suspension was stained with FITC anti-gC antibody. Each point represents mean cell number ± SEM per 3 mice corneas. Experiments were repeated three times. Panels: A) gC expression in cornea of infected mice: and B) Virus titer in the eye of infected mice.