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. 2019 Mar 22;15(3):e1007667. doi: 10.1371/journal.ppat.1007667

Fig 7. HIRA is required for efficient ISG expression during HSV-1 infection.

Fig 7

HFt cells were stably transduced to express non-targeting control (shCtrl) or HIRA-targeting (shHIRA) shRNAs. Cells were stimulated with IFN-β or infected with 1 PFU/cell of WT or ΔICP0 HSV-1 for 9 or 17 h (as indicated). (A, B) qRT-PCR quantitation of Mx1, ISG54, ISG15, and OAS1 mRNA levels in shCtrl or shHIRA cells infected with WT or ΔICP0 HSV-1 (as indicated) at 9 or 17 hpi (A and B, respectively). n = 3, means and SD shown. Values expressed relative to shCtrl + IFN-β at either 9 or 17 h (1; dotted line). *** P < 0.001; two-tailed t-test. (C) shCtrl or shHIRA cells were mock infected or infected with 1 PFU/cell of WT or ΔICP0 HSV-1 for 17 h. Whole cell lysates were analysed by western blot for HIRA, viral IE proteins (ICP0, ICP4), ISG (Mx1, ISG54, ISG15) expression levels, and actin (as a loading control). Molecular mass markers are shown. (D) Quantitation of ISG expression levels (as in C). n = 4, means and SD shown. Values normalized to their respective actin loading controls and expressed relative to their normalized levels in mock infected shCtrl cells (1; dotted line). * P < 0.05, ** P < 0.01, *** P < 0.001, ns (not significant); two-tailed t-test.