Figure 2.

Gle2 has a role in cell cycle regulation. (a) Deletion of GLE2 delays cell cycle progression. Flow cytometric analysis of wild type and gle2∆ cells after arrest with α‐factor (top). The percentage of cells with a haploid (1 N) or diploid (2 N) genome was calculated (bottom). (b) Deletion of GLE2 causes chromosome missegregation. Loss rates relative to wild type (top) and loss rates per cell division are depicted (bottom). mad1∆, defective in the spindle attachment checkpoint served as a positive control. (c) Nuclear mRNA export defects in gle2∆ are weak, when compared to the mRNA export mutant rat7–1 (nup159). Poly(A)⁺‐containing RNA was stained with a Cy3‐labelled oligo d(T)₅₀ probe (red); DNA was stained with Hoechst (blue) in fluorescence in situ hybridization experiments.