Figure 3.

Gle2 is needed for correct formation of the septin ring. (a) Drop dilution test shows genetic interactions of gle2∆ with all septin mutants. (b) The temperature sensitive phenotype of the cdc10–1 mutant, regarding cell size and shape, is drastically enhanced when combined with a deletion of GLE2. (c) Quantification of the average cell length of the strains shown in (b). (d) Western blots of co‐immunoprecipitations (co‐IPs) show interactions of Cdc10 with Gle2. Rps3 served as a negative control. (e) Interaction of the septin ring components Cdc10 and Cdc11 is disturbed in gle2∆ cells as shown by western blots of co‐IPs between the septins. (f) Quantification of three different experiments shown in (e). (g) Cdc10‐GFP and Cdc11‐GFP are drastically mislocalized from the bud neck to the bud tip in strains deleted for GLE2. (h) Quantification of three different experiments shown in (g).