Figure 4.

The cell cycle regulating GTPase Cdc42 requires Gle2 for correctly timed localization. (a) Drop dilution tests uncover genetic interaction of gle2∆ with mutant alleles of CDC42 and the major cell cycle kinase CDC28 (CDK1). (b) Co‐immunoprecipitation and western blot experiments reveal physical interaction of Cdc42 and Cdc28 with Gle2. (c) GFP‐microscopy during a time course experiment with synchronized cells show a prolonged presence of Cdc42 at the bud tip in gle2∆ cells. (d) Quantification of three different experiments shown in (c). A minimum of 100 cells was counted for each time point. (e) Average bud length of cells shown in (c) was determined and reveals significant elongation for cells lacking GLE2.