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. 2019 Apr 18;14(4):e0215441. doi: 10.1371/journal.pone.0215441

Fig 2. Illumina sequence analyses demonstrate enrichment of the protected 12 kb lambda DNA region.

Fig 2

Plots of aligned Illumina reads of the protection experiment mapped to the lambda target genome using BWA 0.7.12-r1039. Sample 1 had no Cas9/sgRNA and no exonuclease treatment with a 120,000:1 copy ratio of lambda to human DNA. Sample 2 had no Cas9/sgRNA and no exonuclease treatment with a 3,000:1 copy ratio of lambda to human DNA. Sample 3 had Cas9/sgRNA Lambda F2 and Lambda R6 treatment with exonucleases III and VII with a 120,000:1 lambda DNA to human genomic DNA copy ratio. Sample 4 had Cas9/sgRNA Lambda F2 and Lambda R6 treatment with exonucleases III and VII with a 3000:1 lambda DNA to human genomic DNA copy ratio.