Fig 5. Illumina and Nanopore sequencing analyses demonstrate enrichment of the target human CFTR genomic protected region.

NGS reads mapping to the CFTR region from an Illumina short read library prepared from Cas9/sgRNA and exonuclease treated human genomic DNA (Sample 1) and a control sample with no Cas9/sgRNA nor exonuclease treatment (Sample 2). Sample 3 is the plot of long-read Nanopore sequencing from a library prepared from Cas9/sgRNA and exonuclease treated human genomic DNA.