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. 2019 Apr 18;14(4):e0215441. doi: 10.1371/journal.pone.0215441

Fig 6. Targets for Cas9/sgRNA multiplexing protection experiments.

Fig 6

(A) Diagram showing the position of two Cas9/sgRNA complexes within the CFTR gene on human chromosome 7, two Cas9/sgRNA complexes within the POLR2a gene on human chromosome 17, and the location of the qPCR probes used in the analyses. (B) qPCR analyses demonstrating multiplexing Cas9/sgRNA protection of human genomic targets. Ratios of on target and off target results for exonuclease treated and non-exonuclease treated samples. Sample 1, no protection; Sample 2, CFTR protection; Sample 3, POLR2a protection; Sample 4, average of four independently performed CFTR/POLR2a multiplex protection reactions. (C) NGS reads mapping to two regions from an NGS library prepared from Cas9/sgRNA and exonuclease treated human genomic DNA for both CFTR 10 kb protection and POLR2a 7 kb protection.