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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Hypertension. 2019 Apr;73(4):910–918. doi: 10.1161/HYPERTENSIONAHA.118.12642

Figure 2: Alpha-synuclein (AS)/tyrosine hydroxylase (TH) colocalization indices in post-mortem sympathetic ganglion tissue.

Figure 2:

Panel A shows representative normalized mean deviation product (nMDP) values and corresponding colocalization counts from sympathetic ganglion tissue of a patient with a Lewy body disease (red outline) and a control subject without Lewy body disease (blue outline). nMDP is defined as the correlation between individual pairs of pixels. Values for nMDP range from −1.0 to +1.0, where +1.0 means perfect correlation and −1.0 means perfect exclusivity. At the bottom of Panel A is the formula used to calculate the colocalization index. Counts for nMDP values above 0.3 were summed, and 0.1 was added to the sum to avoid taking the log of zero when the sum of counts was zero. If there were no AS/TH colocalization, the index would be −1.0 (the log of 0.1). Panel B shows individual AS/TH colocalization indices from 8 patients with a Lewy body disease (red) and 7 control subjects without a Lewy body disease (blue). Panel C shows a scatterplot relating individual values for the AS/TH colocalization index as a function of the log of the apical myocardial concentration of norepinephrine (NE), expressed in units of pmol/mg wet weight, in Lewy body nOH patients (red), non-Lewy body nOH patients (blue), and control subjects without either nOH or a Lewy body disease (gray). Note complete separation of the Lewy body nOH patients from the non-Lewy body nOH patients and control subjects in terms of both AS/TH colocalization indices and myocardial NE contents.