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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Arthritis Rheumatol. 2019 Jan 5;71(2):244–257. doi: 10.1002/art.40696

Figure 2. Lymphatic endothelial cells in PTOA synovium have an inflammatory phenotype.

Figure 2.

Mice received MLI or sham surgery as in Fig.1. (A) LECs were isolated from synovium at 6 weeks post-surgery with anti-PDPN antibody, and the enrichment of this PDPN+ population was confirmed by flow cytometry (n=6 mice). (B) RNAseq was performed on the PDPN+ cells, which revealed about 1,000 differentially expressed genes in PTOA vs. sham synovial LECs. Pathway analysis indicated 12 dysregulated pathways (# indicates pathways involving NF-κB signaling; n=4–5 mice/group). (C) Synovial LECs from a different cohort of PTOA mice at 6 weeks post-surgery were subjected to qPCR. Data are mean + SD in which the Sham expression level = 1 (n=5 mice/group; *p<0.05 via unpaired t test).