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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Anal Bioanal Chem. 2019 Mar 11;411(12):2729–2741. doi: 10.1007/s00216-019-01713-5

Fig. 5.

Fig. 5

Concentration-dependent correlation between intracellular peptide distribution and CPP uptake efficiency. A coefficient of variance (COV) was calculated for each individual cell analyzed as a metric of intracellular heterogeneity to determine the effect of peptide concentration on intracellular distribution. (a) Population of fluorescent intensity signals for HeLa cells incubated with 10 μM OWRWR demonstrating a logarithmic normal distribution (n=539, r2=0.80, p<0.05) (b) Observed intracellular heterogeneity (COV) of 10 μM OWRWR peptide solution in HeLa cells demonstrating normal distribution (n=539, r2=0.65, p<0.05) (c) Correlation between intracellular heterogeneity and fluorescent for the entire population of HeLa cells incubated with four peptides at the two concentrations numerically simulated with exponential associations. (d) Comparison of the median values of the intracellular heterogeneity and the median cellular fluorescence intensity for all conditions.