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Coomassie blue stain of exoproteins isolated from wild-type (lane 1) and mutant strains, pho34B12 (lane 2), ORF1* (lane 3), and ORF2* (lane 4). Extracellular proteins were isolated from equal amounts of wild-type and mutant bacterial cells grown in Luria–Bertani media (OD600 = 2.5–3.0), separated on a 4–20% gradient polyacrylamide gel, and stained with Coomassie brilliant blue R-250.
