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. 2019 Apr 12;9:262. doi: 10.3389/fonc.2019.00262

Figure 4.

Figure 4

DEPDC1 promoted cells proliferation and drived cell cycle transition in breast cancer cells. (A) Expression of DEPDC1 in MCF-7 and MDA-MB-231 cells was determined using RT-PCR. (B) DEPDC1 was stably overexpressed in MCF-7 cells and silenced in MDA-MB-231 cells by transfection and selection. DEPDC1 expression was confirmed by western blot analysis. (C,D) Viability of breast cancer cells after being processed was evaluated using CCK-8 assay, which suggested DEPDC1 promoted the proliferation of breast cancer cells. (E,F) Cycle distribution analysis of breast cancer cells with altered DEPDC1 expression were performed by flow cytometry. (G) Enrichment plots of gene expression signatures for cell proliferation and cell cycle (H) were analyzed according to DEPDC1 mRNA expression level by GSEA of TCGA_BRCA dataset. False discovery rate (FDR) gives the estimated probability that a gene set with a given normalized ES (NES) represents a false-positive finding; FDR <0.25 is a widely accepted cutoff for the identification of biologically significant gene sets.