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. 2019 Apr 12;10:227. doi: 10.3389/fendo.2019.00227

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Copyright © 2019 Nakamachi, Tanigawa, Konno, Shioda and Matsuda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Brain distribution of zebrafish PACAP and PAC1R mRNA. Schematic diagram of brain region in zebrafish was shown in (A). Distribution and expression of PACAP mRNA, adcyap1a and adcyap1b genes (B), PAC1R mRNA, adcyap1r1a and adcyap1r1b genes (C) were determined using absolute quantitative real-time PCR. The y-axis is displayed on a logarithmic scale. n = 5 in each brain region. Abbreviations: TC, telencephalon; OT, optic tectum; DC, diencephalon; CB, cerebellum; BS, brain stem; SC, spinal cord.