Table 1. Number of follicles and recovered oocytes in non-stimulated (control) and superstimulated Japanese Black cows.
| Non-stimulated | Superstimulated | ||
|---|---|---|---|
| No. of OPU sessions | 7 | 6 | |
| No. of follicles at OPU | |||
| Large follicles (> 8 mm) | 1.1 ± 0.3 ** | 40.2 ± 4.2 ** | |
| Medium follicles (≥ 5 to ≤ 8 mm) | 3.3 ± 0.7 * | 15.5 ± 6.4 * | |
| Small follicles (< 5 mm) | 47.9 ± 8.9 ** | 7.5 ± 2.8 ** | |
| Total follicles | 52.3 ± 8.9 | 63.2 ± 12.5 | |
| No. of oocytes | |||
| Normal oocytes (grade 1 to 3) | 29.0 ± 4.1 | ||
| Abnormal oocytes (grade 4 and 5) | 5.4 ± 1.4 | ||
| Oocytes with expanded cumulus cells | 36.0 ± 7.0 | ||
| Oocytes with non-expanded cumulus cells | 8.3 ± 1.7 | ||
| Degenerated oocytes | 2.0 ± 1.4 | ||
| Total oocytes recovered | 34.4 ± 3.7 | 46.3 ± 9.2 | |
| Recovery rate of oocytes (%) a | 69.8 ± 5.6 | 73.8 ± 2.9 | |
Oocytes were collected from non-stimulated and superstimulated cows prior to ovum pick-up (OPU). Data are expressed as means ± SEM per head per OPU session. a The percentages of total number of oocytes recovered to total number of follicles was denoted. Asterisks indicate that values within the same row are significantly different (*, P < 0.05; **, P < 0.01).