Table 3. In vitro development of embryos produced using in vitro- (non-stimulated, control) and in vivo- (superstimulated) matured oocytes.
| Non-stimulated | Superstimulated | |
|---|---|---|
| No. of putative zygotes cultured | 200 | 204 |
| No. of embryos cleaved (%) a | 166 (83.0) | 160 (78.4) |
| No. of normally cleaved embryos (%) b | 86 (51.8) * | 105 (65.6) * |
| No. of embryos developed to blastocyst stage on Day 8 (%) b | 100 (60.2) | 96 (60.0) |
| No. of IETS code 1 and 2 blastocysts (%) b | 81 (48.8) | 92 (57.5) |
| No. of IETS code 1 blastocysts (%) b | 42 (25.3) * | 59 (36.9) * |
| No. of IETS code 1 and 2 blastocysts from normal cleaved embryos (%) b | 49 (29.5) ** | 73 (45.6) ** |
Oocytes collected from non-stimulated and superstimulated Japanese Black cows were subjected to in vitro and in vivo maturation in the non-stimulated and superstimulated groups, respectively, and used for in vitro fertilization. Data for the non-stimulated and superstimulated groups were obtained from 7 and 6 replicates, respectively. a The percentages of cleaved embryos at 50 hpi to cultured zygotes was denoted. b The percentages of embryos corresponding to each criterion in cleaved embryos was denoted. Asterisks indicate that values within the same row are significantly different (*, P < 0.05; **, P < 0.01).