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. 2019 Jan 31;65(2):163–170. doi: 10.1262/jrd.2018-104

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©2019 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 1. — Effects of LBP on development of previously-cryopreserved murine two-cell embryos. (a) Effects of LBP on blastocyst attainment rate. (b) Effects of LBP on total per-blastocyst cell numbers. F: fresh two-cell embryos; V: previously-cryopreserved (vitrified) two-cell embryos; V+Number (LBP dose, μg/ml): previously-cryopreserved (vitrified) two-cell embryos cultured in the presence of different LBP doses. Results represent means ± SEM of three independent experiments. Error bar letters denote significant differences (P < 0.05).