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. 2019 Jan 31;65(2):163–170. doi: 10.1262/jrd.2018-104

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©2019 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 2. — Effects of LBP on mitochondrial distribution and MMP in previously-cryopreserved murine two-cell embryos after 2 h culture. (a and b) Effects of LBP on MMP. (c) Effects of LBP on mitochondrial distribution. Fresh: fresh two-cell embryos; vitrified: previously-cryopreserved (vitrified) two-cell embryos; Vit + LBP: previously-cryopreserved (vitrified) two-cell embryos cultured in the presence of 200 μg/ml LBP; Fre + LBP: fresh two-cell embryos cultured in the presence of 200 μg/ml LBP. Results represent means ± SEM of three independent experiments. Error bar letters denote significant differences (P < 0.05).