Fig. 3.

Localization of WGA immunoreactivity and tandem dimer Tomato (tdTomato)-expressing kisspeptin neurons of the ARC and AVPV in a heterozygous Kiss1-tdTomato female rat injected with WGA into the 4V. Representative photomicrographs of the brain sections including ARC (A) or AVPV (B) visualized by immunohistochemistry for WGA (green) and tdTomato fluorescence (red). Right columns indicate the number of tdTomato-expressing cells with (gray) or without WGA immunoreactivity (open). Values are indicated as means ± SEM. Scale bars = 100 μm.