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. 2019 Jan 19;65(2):147–153. doi: 10.1262/jrd.2018-147

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©2019 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 2. — Detection of neurotensin receptor 2 (NTR2) expression in bovine sperm. (A) NTR2 expression in bovine sperm was evaluated by western blot. (B) Representative immunocytochemical image showing NTR2 localization in bovine sperm. Note the spot-like immunoreactivity detected at the tail of the sperm. Blue: nuclei (Hoechst 33342). Green: NTR2. Bars = 10 μm.