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. 2019 Apr 12;10:718. doi: 10.3389/fmicb.2019.00718

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Copyright © 2019 Park, Ávila-Pérez, Madere, Hilimire, Nogales, Almazán and Martínez-Sobrido.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Aurintricarboxylic acid inhibition of ZIKV replication: Vero (A) and A549 (B) cells (24-well plate format, 2.5 × 10⁵ cells/well, triplicates) were infected (MOI 0.1) with Paraiba/2015. Tissue culture supernatants were collected at 24, 48, and 72 h p.i., and viral titer were calculated by immunostaining (fluorescent forming units, FFU/ml). Dotted line indicates the limit of detection (20 FFU/ml). Data was expressed as mean and standard deviations (SD) from three independent experiments conducted in triplicates. Statistical analysis was conducted by two-way ANOVA, ^∗p < 0.0332, ^∗∗p < 0.0021, ^∗∗∗p < 0.0002, ^∗∗∗∗p < 0.0001, or no significance (n.s.).