FIGURE 6.

Aurintricarboxylic acid inhibition of African and Asian/American ZIKV strains: Vero cells (96-well plates, 5 × 10⁴ cells/well, triplicates) were infected with 25 PFU of Uganda/1947 (A), Nigeria/1968 (B), Puerto Rico/2015 (C), and French Polynesia/2013 (D) ZIKV strains. After 1 h of viral adsorption, the indicated concentrations (twofolds dilutions, starting concentration 1,000 μM) of ATA were added to 100 μl of infection media containing 1% of Avicel. Infected cells were fixed for virus titration by immunostaining assay at 36–60 h p.i., depending on the ZIKV strains. Dotted line indicates 50% inhibition. Data was expressed as mean and SD from three independent experiments conducted in triplicates.