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. 2019 Apr 12;10:757. doi: 10.3389/fimmu.2019.00757

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Copyright © 2019 Liubomirski, Lerrer, Meshel, Rubinstein-Achiasaf, Morein, Wiemann, Körner and Ben-Baruch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CXCL8, CCL2 and CCL5 reach their highest expression levels when TNBC:CAF “Contact” co-cultures are stimulated by TNFα and IL-1β. The Figure demonstrates similar experiments and statistical analyses as those of Figure 3, performed herein with MDA-MB-231 cells and CAFs, using similar cytokine concentrations. (A) Activation of p65 and JNK. (B) CXCL8 (B1), CCL2 (B2) and CCL5 (B3) expression levels. In all parts of the Figure, the results are of a representative experiment of n ≥ 3 independent experiments. *, **, ***, # - As described in Figure 3. ns=non-significant.