Skip to main content
. 2019 Apr 12;10:757. doi: 10.3389/fimmu.2019.00757

Figure 8.

Figure 8

The pro-angiogenic activities of factors released by tumor:MSC “Contact” co-cultures are promoted by TNFα in TNBC but not in luminal-A cells. Studies of endothelial cell (HPMEC) migration in response to CM derived from different cell combinations of MDA-MB-231 cells (“MDA”) (A) and MCF-7 cells (B). (A1, B1) Representative photos of HPMEC migration in response to TNFα-free CM derived from TNFα-stimulated tumor:MSC “Contact” co-cultures (10 ng/ml), from tumor cells alone, from tumor cells stimulated by TNFα alone and from tumor cells grown under “Contact” conditions with MSCs only. Bars, 50 μm. (A2, B2) Migrated HPMEC were counted in multiple photos per insert of the experiments presented in A1 and B1. ***p < 0.001, *p < 0.05, ns=non-significant for comparisons between CM of different cell combinations and CM of tumor cells treated by vehicle. Photos and their quantifications are representatives of n ≥ 3 independent experiments, performed with MSCs of 3 different donors.