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. Author manuscript; available in PMC: 2019 Apr 19.
Published in final edited form as: Bioorg Med Chem. 2014 Jun 30;22(17):4910–4916. doi: 10.1016/j.bmc.2014.06.050

Figure 3.

Figure 3.

Inhibition of recombinant eEF-2K activity by compounds. Inhibitor dose response assays were performed with 5 nM eEF-2K enzyme at various concentrations of the inhibitor, in the presence of 50 μM free Ca2+, 200 nM CaM and 10 μM [γ−32P]ATP, against 150 μM peptide substrate as described under the experimental section. The inhibitor was incubated with eEF-2K for 30 min at 30 °C before initiating the assay with peptide and [γ−32P]ATP. Data are plotted as the percentage of kinase activity as a function of inhibitor concentration, and fit to equation 1. (A) Compound 6. (B) Compound 9.