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. Author manuscript; available in PMC: 2019 Apr 19.
Published in final edited form as: Bioorg Med Chem. 2014 Jun 30;22(17):4910–4916. doi: 10.1016/j.bmc.2014.06.050

Figure 4.

Figure 4.

Cellular analysis of compounds against eEF-2K activity. MDA-MB-231 tumorigenic breast cells were treated with 75 μM compound 6, 9, or 12 for 1 h in low-glucose media ± 2-DOG, along with the appropriate controls, as described under the experimental section. One set of samples was stimulated with 25 mM 2-DOG (30 min) to activate the AMPK-eEF-2K axis. Cell lysates were then analyzed by Western blotting using antibodies specific for phospho-eEF-2 (Thr-56), total eEF-2, total eEF-2K and pan-Actin as described under the experimental section.