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. 2019 Apr 12;10:754. doi: 10.3389/fimmu.2019.00754

Figure 2.

Figure 2

CP growth requires adequate nutrition available to the host. (A) WT, Atg16l1+/−, and Atg5−/− MEFs were seeded at 5 × 104 in a 24-well plate. Total cell numbers were counted after 3 days. (B,C) Atg5−/− MEFs were infected with CP (MOI 10) and treated with 1 μg/ml cycloheximide. Inclusions were counted 68 h post infection (B), as was inclusion size (C). (D) WT, Atg16l1+/−, and Atg5−/− MEFs were seeded at the indicated amounts in a 24-well plate and infected with CP (MOI 10). Inclusions were counted 68 h post infection. (E) Atg5−/− MEFs were infected with CP (MOI 10) and the media was supplemented with either MEM amino acids (AA), Na-pyruvate (NaPyr), or both. Inclusions were counted 68 h post infection. All panels are representative of three experiments. Data are mean ± SEM. Significance was assessed by students t-test (B,C) or one-way ANOVA (A,E). *p < 0.05, ***p < 0.001, **** p < 0.0001.