Figure 5.

Autophagy induction fails to rescue mice from CP infection. WT MEFs were infected with CP (MOI 10) and treated with 10 μM Tamoxifen during the first 2 h of infection. (A) CP inclusions. (B) CP progeny. (C) C57Bl/6 mice were infected with 2 × 10⁶ IFU CP. Six hour prior to infection, mice received either sham or 3 mg/kg rapamycin i.p., and again every other day. Mortality was assessed (n = 5–9). (D–J) C57Bl/6 mice were infected with 1 × 10⁶ IFU CP. Six hour prior to infection, mice received either sham or 3 mg/kg rapamycin i.p., and again every other day (n = 8–12, pooled from two separate experiments). Mice were sacrificed on days 3, 6. (D) Total cells in the BALF. (E) BALF Alveolar macrophages. (F) BALF neutrophils. (G) BALF DCs. (H) BALF T-cells. (I) Bacterial burden in the lungs. (J) IL-1β in the BALF after CP infection and rapamycin treatment. (K) Lung inflammation score on day 6. Significance was assessed by students t-test. Survival curves was compared using the Log-Rank (Mantel-Cox) Test. Data are mean ± SEM. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.