Figure 4.

Expression of Gli1 from the Gli2 locus did not change neural tube patterning in the absence of Smo. Transverse sections of E10.5 embryos processed for immunofluorescence analyses. (A–C) Foxa2 was expressed in the floor plates of the wild type (A) and Gli2^1ki/1ki (B), but not in the Gli2^1ki/1ki;Smo^−/− (C) neural tubes. (D–F) Nkx2.2 labeled V3 interneurons in the wild type (D) and Gli2^1ki/1ki (E), but was absent in Gli2^1ki/1ki;Smo^−/− (F) neural tubes. (G–I) Olig2 labels motor neuron progenitors in the wild type (G) and Gli2^1ki/1ki (H), but not in Gli2^1ki/1ki;Smo^−/− (I) neural tubes. (J–L) Nkx6.1 was expressed in the wild type (J) and Gli2^1ki/1ki(K), but not in Gli2^1ki/1ki;Smo^−/− (L) neural tubes. (M–O) Pax7 was expressed in the dorsal region of the wild type (M) and Gli2^1ki/1ki(N), and was expanded ventrally in the Gli2^1ki/1ki;Smo^−/− (O) neural tubes. Dashed lines outline the neural tubes and brackets mark the expression domains. Note the Gli2^1ki/1ki;Smo^−/− images are of higher magnification to show the details of the neural tubes that are significantly smaller than those of their littermates. n = 3 embryos for each genotype.