Fig. 3.
Therapeutic efficacy of HB1.F3.CD and HB1.F3.CD.IFN-β with prodrug 5-fluorocytosine (5-FC) in vitro. Twenty-four hours after A375SM cells (4×103 cells/well) were seeded in 96-well plates, human neural stem cells (hNSCs) were seeded in the same well and all wells were treated with 5-FC for 3 days. The viability of the A375SM cells was measured using an MTT assay. (A) The cell viability of various concentrations of 5-FC and 5-fluorouracil (5-FU) on A375SM cells. (B) The therapeutic effect of hNSCs with 5-FC treatment at increasing concentrations (100, 200, 300, 400, and 500 μg/mL) on the A375SM co-culture system. Phosphate buffered saline (PBS) was used as a negative control. Data were represented as mean±standard error of mean. *p < 0.05 vs. control.