Table 1.
Controlled delivery of nonviral vectors via supramolecular-based CD hydrogels.
| Polymers/CDs | Vectors | Outcomes | Approaches | Targets | References |
|---|---|---|---|---|---|
| PF68-PLL/α-CD | pDNA-GFP | Sustained pDNA delivery for 80 h; transfection efficiency ~14% | mouse fibroblast cells 3T3 | n.s. | [9] |
| MPEG-PCL-PDMAEMA/α-CD | pDNA-luc | Sustained release of pDNA up to 6 days; transfection efficiency comparable to freshly prepared PEI polyplexes | COS-7 cells | n.s. | [13] |
| PEG-α-CD-cross-linked PVDT | pDNA-luc | Efficient reverse gene transfection of cells cultured on the gel surface | COS-7 cells | n.s. | [39] |
| MPEG-PCL-PEI/α-CD MPEG-PCL-PEIFA/α-CD | pDNA-GFP pDNA-Nur77 |
Sustained release of pDNA for 7 days; transfection efficiency of 63% at optimal weight ratio of 1.5; significant inhibition of therapeutic resistant tumor growth with high expression of Bcl-2 proteins Higher efficiency when combining the chemotherapeutic agent paclitaxel and the targeting ability of FA |
HEK293 cells, tumor model (BALB/c nude mice) | tumor | [15,40] |
| MPEG-PLLD-Arg/α-CD | pMMP-9 | Controlled release for 6 days; transfection efficiency up to 72%; sustained tumor growth inhibition after 21 days with good biocompatibility | HNE-1 cells, nude mice bearing HNE-1 tumors | tumor | [37] |
Abbreviations: CD: cyclodextrin; Pluronic® F68; PLL: poly(l-lysine); α-CD: alpha-CD; MPEG-PCL-PDMAEM:. methoxy-poly(ethylene glycol)-b-poly(ε-caprolactone)-b-poly[2-(dimethylamino)ethyl methacrylate]; PEG: poly-ethylene glycol; β-CD: beta-CD; PVDT: poly-2-vinyl-4,6-diamino-1,3,5-triazine; PEI: poly(ethylene imine); PLLD-Arginine-functionalized PLL dendron; pDNA: plasmid DNA; GFP: green fluorescent protein; luc: luciferase; Nur 77: Bcl-2 (B-cell lymphoma 2) conversion Nur77 gene; FA: folic acid; MMP-9: matrix metalloproteinase 9; HNE-1: human nasopharyngeal carcinoma HNE-1 cells; n.s.: not specified.