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Immunization and sample collection schedule. The immunization was given two times mucosally with a 12-weeks interval. Rectal biopsies were collected before and at day 3 and 21 post immunizations, Blood was collected before and at day 7 post immunizations. Axillary LNs were collected before the immunization and Inguinal LNs were collected at day 14 after the 2nd immunization.
