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. 2019 Apr 12;10:779. doi: 10.3389/fimmu.2019.00779

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Copyright © 2019 Ko, Helmold Hait, Enyindah-Asonye, Rahman, Hoang and Robert-Guroff.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DC frequencies in Ad-SIV immunized group are positively correlated with antigen-specific T and B cell responses in rectal mucosa. Non-parametric Spearman test was performed for correlation analysis by using the data obtained after 1st Ad-SIV (A–C) and 2nd Ad-SIV (D–F) immunizations. Frequencies of Ad-SIV immunized rectal DCs were measured at day 3 after each immunization. Rectal antigen-specific T and B cell responses in Ad-SIV group were measured at day 21 after each immunization. SIV Gag, SIV_M766 Env, or SIV_CG7V Env peptide pools as indicated were used for 6 h of T cell stimulation. The antigen-specific responses are reported following subtraction of unstimulated values.