Table 3.

Enzyme cocktail for in vitro digestion, incubation parameters, and conditions for sample preparation.

Two Phase In-Vitro Digestion	Enzyme Cocktail Solution	Buffer Solution	pH Conditions	Incubation Conditions
Gastric Phase	0.9% Saline Solution and 4 g/L pepsin from porcine gastric mucosa in 0.1 M Hydrochloric acid.	5 M HCL	pH = 2.0	Incubation with orbital shaking at 37 °C and 95 rpm for 1 h
Intestine Phase	2 g/L pancreatin from porcine pancreas and 12 g/L bile extract in 0.1 M NaHCO3	0.9 M solution of NaHCO3, 2 M NaOH	Initial pH = 5.3 Final pH = 7.5	Incubation in orbital shaking at 37 °C and 95 rpm for 2 h