Figure 5.
Hepatic β-oxidation in C57BL/6 mice fed high fat/high sucrose diets with casein (19 E%) or casein (9.5 E%) combined with different CPHs (9.5 E%) for 12 weeks. (A) In vitro β-oxidation of palmitoyl-CoA in fresh liver homogenates. (B) In vitro activity of acyl-CoA oxidase 1 (ACOX1) in frozen liver homogenates. Mean values with standard deviation are shown (n = 5–6). (C) Hepatic gene expression of carnitine palmitoyl transferase 1a (Cpt1a), Cpt2, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta (Hadhb), and 2,4-dienoyl-CoA reductase 1 (Decr1). Mean values with standard deviation are shown (n = 7). Significant differences between values were determined by one-way ANOVA with Fisher’s LSD test (* p < 0.05).
