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. 2019 Apr 19;20:71. doi: 10.1186/s13059-019-1671-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Schematic overview of the BRB-seq protocol. This schema highlights in details all steps of the final BRB-seq protocol. The bottom grayed window shows the final BRB-seq construct used for Illumina sequencing. The read Read1 and Read2 primers are used to sequence the barcode/UMI and cDNA fragment respectively. Index read (i7) is used to demultiplex Illumina libraries. Legend: DS cDNA, double-stranded cDNA