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. 2019 Apr 12;10:797. doi: 10.3389/fimmu.2019.00797

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Copyright © 2019 Bucher, Schlundt, Wulsten, Sass, Wendler, Ellinghaus, Thiele, Seemann, Willie, Volk, Duda and Schmidt-Bleek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Torsional testing of mouse femora with different immune phenotype. Keeping mouse under non-SPF conditions increased the torsional stiffness compared to 12 months old SPF mice. Yield torque and maximal torque increased by age and was further affected by non-SPF housing. The post-yield displacement (PYD) revelaed a more brittle fracture manner at 12 months under non-SPF compared to 12 months under SPF housing, the 3 month old bone fractured in a more ductile manner. N = 6 per group, boxplot distribution with median, Mann-Whitney U-test, ^*p < 0.05.