Figure 2.

(A) Editing and alternative splicing of 5HT_2C receptor pre-mRNA. Schematic representation of the serotonin receptor 5HT_2C pre-mRNA, with the positions of the major sites for RNA editing (A–E) depicted. The positions of alternative splicing affecting the same region are indicated. Splicing of intron 3 leads to the long splice variant (LF), resulting in a functional receptor including the edited residues. Alternative splicing with partial deletion of exon 3 gives rise to the short splice form (SF), which is translated to yield an inactive receptor molecule and also lacks the edited sites. (B) Ratios of the 5HT_2C splice forms in control human brain (cortex, WM A) versus glioma tissues (GBM1, 2, 6) and glioma-derived cell lines (GC1, 2). Percent values for the truncated variant (SF) and functional variant (LF) are determined by semiquantitative RT-PCR.