Skip to main content
. 2018 Mar 22;16(5):460–472. doi: 10.1038/s41423-018-0003-5

Fig. 8. DAP12 stabilizes TREM-1 surface expression and multimerization.

Fig. 8

a Normalized DAP12 mRNA and protein quantification in native and DAP12-silenced conditions. b–d Isolated human primary neutrophils and monocytes were incubated 12 h with siRNAs and incubated during 24 h in resting conditions or stimulated with LPS (100 ng/ml). b TREM-1 expression on monocytes. c TREM-1 expression on neutrophils. d TREM-1/TREM-1 interactions (red blobs) and nucleus (blue) on monocytes after 24 h by confocal microscopy (scale bar: 10 µm). Data information: data are representative of at least three different experiments. ns nonsignificant. *p < 0.05, **p < 0.01, ***p < 0.001 vs. resting or as indicated, as determined by the two-tailed Student’s t-test