Fig. 8.

In vivo transient Opn knockdown rescues animals from APAP-induced death. a Schematic of in vivo transient Opn knockdown combined with APAP overdose. b mRNA levels of hepatic Opn in mice at indicated time points after siOPN treatment (n = 2). c Survival was followed for 60 h after administration of a lethal dose of APAP (n = 10). d Serum ALT levels at the indicated time points after APAP treatment (300 mg/kg, i.p, n = 4–5). e H&E staining in liver sections (n = 4–5, origin magnification ×100). f Western blot of the hepatic CYP2E1 levels 2 h after APAP challenge. β-actin was used as a loading control. Quantification of the relative hepatic CYP2E1 levels in NC or siOPN-treated mice (n = 3). g IHC of cleaved caspase-3 in the livers of NC or siOPN-treated mice 24 h after APAP challenge (origin magnification ×100). Data are shown as the means ± SEM, *P < 0.05. NC negative control