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. 2019 Jan 14;13(5):1168–1182. doi: 10.1038/s41396-018-0338-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Depletion of isoprene (a) and reciprocal production of total methylbutenes (b) in anaerobic cultures containing an enriched isoprene reducing culture amended with H₂ and HCO₃⁻ with and without isoprene. Cultures amended with isoprene produced 40% less acetate (c) while still consuming the same amount of H₂ (d). Acetobacterium cells ml⁻¹ (e) calculated from 16S rDNA gene copies ml⁻¹ demonstrate equivalent growth in both conditions. ***p-value < 0.0001, **p-value = 0.005 analyzed by a two-way ANOVA. Error bars represent one standard deviation (n = 4)