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. 2019 Jan 15;13(5):1209–1225. doi: 10.1038/s41396-019-0346-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Transmission electron microscopy images of H. (S.) methanophila. a Symbiotic methane-oxidizing bacteria (MOX) are abundant in the mesohyl, particularly in regions close to the choanocyte chambers (chambers not visible in image), sp = sponge cells, n = nucleus. b High-resolution image of white box labeled b from panel (a), showing intracellular membranes (arrows) typical for MOX. The MOX at the lower half of the image are dividing; bc = bacteria with a different morphology than the MOX symbionts. c MOX symbiont in a lysosome of an amebocyte, l = lysosome. d Mesohyl with an embryo surrounded by symbiotic MOX, and a bacteriocyte containing bacteria with a different morphology than the symbiotic MOX (bc). e MOX symbionts within an embryo. he = heterogeneous yolk. f A bacteriocyte containing bacteria with a different morphology than the MOX symbionts. bc = bacteria; n = nucleus