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. 2019 Jan 15;13(5):1252–1268. doi: 10.1038/s41396-019-0348-5

Table 1.

A summary of common proteins identified from cell-extracts of isophthalate- and benzoate-grown cells of S. aromaticivorans

Gene locia IMG gene annotation and descriptionb pIc Mr (kDa)d MS/MS identification score Coveragee (%)
Isophthalate Benzoate
0938 Aldehyde:ferredoxin oxidoreductase 5.87 76.5 7139 5845 76
0937 Fe-S-cluster-containing dehydrogenase component 5.11 20.1 198 111 27
0936 Tungsten-dependent benzoyl-CoA reductase bamD 6.46 43.6 1498 1399 43
0935 4Fe-4S binding domain-containing protein 5.30 54.9 1647 1375 39
0934 Putative adenylylsulfate reductase ET protein QmoB 6.05 63.2 1680 1945 60
0933 Coenzyme F420-reducing hydrogenase, delta 8.19 31.3 629 711 33
3491 Subunit 7.18 63.9 498 585 29
0912 NADH-quinone oxidoreductase subunit F 6.16 62.1 1034 1588 41
1236 Cyclohexa-1,5-dienecarbonyl-CoA hydratase 6.25 28.4 667 1091 69
1234 6-Hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase 7.24 39.8 5147 4647 74
1235 6-Ketocyclohex-1-ene-1-carbonyl-CoA hydrolase 6.90 42.7 11,667 16,783 84
1233 3-Hydroxy acyl-CoA dehydrogenase 8.35 26.8 4335 4710 75
3465 Acetyl-CoA C-acetyltransferase 6.05 45.3 4853 4808 72
1516 Acetyl-CoA C-acetyltransferase 6.19 42.0 9503 9230 92
1412 Acetyl-CoA C-acetyltransferase 6.90 41.5 6858 8428 89
2257 Glutaryl-CoA dehydrogenase (decarboxylating) 7.46 63.5 21,884 24,664 84
1512 Glutaryl-CoA dehydrogenase (non-decarboxylating) 6.13 42.3 21,884 24,664 84
1515 3-Hydroxybutyryl-CoA dehydratase/enoyl-CoA hydratase 5.60 27.4 4730 4390 70
1514 3-Hydroxybutyryl-CoA dehydrogenase 6.83 31.7 950 1144 53
1015 Acyl-CoA synthetase 6.29 58.9 164 122 14
1198 Butyryl-CoA dehydrogenase 8.22 42.1 771 316 26

aIMG gene locus tag SynarDRAFT_“0000” from the draft genome of S. aromaticivorans strain UI; bGene annotation and predicted functions of gene products; cpI—isoelectric point; dMass calculated by MS/MS measurement and eCoverage represents the extent of peptides measured during MS/MS identification of respective protein