Table 1.
A summary of common proteins identified from cell-extracts of isophthalate- and benzoate-grown cells of S. aromaticivorans
Gene locia | IMG gene annotation and descriptionb | pIc | Mr (kDa)d | MS/MS identification score | Coveragee (%) | |
---|---|---|---|---|---|---|
Isophthalate | Benzoate | |||||
0938 | Aldehyde:ferredoxin oxidoreductase | 5.87 | 76.5 | 7139 | 5845 | 76 |
0937 | Fe-S-cluster-containing dehydrogenase component | 5.11 | 20.1 | 198 | 111 | 27 |
0936 | Tungsten-dependent benzoyl-CoA reductase bamD | 6.46 | 43.6 | 1498 | 1399 | 43 |
0935 | 4Fe-4S binding domain-containing protein | 5.30 | 54.9 | 1647 | 1375 | 39 |
0934 | Putative adenylylsulfate reductase ET protein QmoB | 6.05 | 63.2 | 1680 | 1945 | 60 |
0933 | Coenzyme F420-reducing hydrogenase, delta | 8.19 | 31.3 | 629 | 711 | 33 |
3491 | Subunit | 7.18 | 63.9 | 498 | 585 | 29 |
0912 | NADH-quinone oxidoreductase subunit F | 6.16 | 62.1 | 1034 | 1588 | 41 |
1236 | Cyclohexa-1,5-dienecarbonyl-CoA hydratase | 6.25 | 28.4 | 667 | 1091 | 69 |
1234 | 6-Hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase | 7.24 | 39.8 | 5147 | 4647 | 74 |
1235 | 6-Ketocyclohex-1-ene-1-carbonyl-CoA hydrolase | 6.90 | 42.7 | 11,667 | 16,783 | 84 |
1233 | 3-Hydroxy acyl-CoA dehydrogenase | 8.35 | 26.8 | 4335 | 4710 | 75 |
3465 | Acetyl-CoA C-acetyltransferase | 6.05 | 45.3 | 4853 | 4808 | 72 |
1516 | Acetyl-CoA C-acetyltransferase | 6.19 | 42.0 | 9503 | 9230 | 92 |
1412 | Acetyl-CoA C-acetyltransferase | 6.90 | 41.5 | 6858 | 8428 | 89 |
2257 | Glutaryl-CoA dehydrogenase (decarboxylating) | 7.46 | 63.5 | 21,884 | 24,664 | 84 |
1512 | Glutaryl-CoA dehydrogenase (non-decarboxylating) | 6.13 | 42.3 | 21,884 | 24,664 | 84 |
1515 | 3-Hydroxybutyryl-CoA dehydratase/enoyl-CoA hydratase | 5.60 | 27.4 | 4730 | 4390 | 70 |
1514 | 3-Hydroxybutyryl-CoA dehydrogenase | 6.83 | 31.7 | 950 | 1144 | 53 |
1015 | Acyl-CoA synthetase | 6.29 | 58.9 | 164 | 122 | 14 |
1198 | Butyryl-CoA dehydrogenase | 8.22 | 42.1 | 771 | 316 | 26 |
aIMG gene locus tag SynarDRAFT_“0000” from the draft genome of S. aromaticivorans strain UI; bGene annotation and predicted functions of gene products; cpI—isoelectric point; dMass calculated by MS/MS measurement and eCoverage represents the extent of peptides measured during MS/MS identification of respective protein