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. Author manuscript; available in PMC: 2019 Apr 19.
Published in final edited form as: Sci Signal. 2018 Oct 30;11(554):eaar3924. doi: 10.1126/scisignal.aar3924

Fig. 2. The RSK inhibitor LJH685 induces relaxation of myogenic tone in mesenteric arteries and suppresses RSK2 phosphorylation in WT smooth muscle cells.

Fig. 2.

(A and B) Tension trace and summary showing relaxation of myogenic tone in response to increasing concentrations of LJH685 in a mesenteric artery in Krebs bicarbonate buffer and pressurized to 60 mmHg. Subsequent treatment with Ca2+-free solution induced maximal vessel dilation. Dimethyl sulfoxide (DMSO) diluent concentrations were matched to LJH685 concentrations. n = 3 to 7 arteries per group. P = 0.04 for DMSO compared to LJH685 treatment, two-way ANOVA. (C) RSK2 Ser227 phosphorylation (normalized to total RSK2) in response to serum application in serum-starved mouse aortic smooth muscle cells or preincubation with LJH685, the PDK1 inhibitor GSK2334470, and the ERK1/2 inhibitor U0126. Rsk2KO smooth muscle cells served as a control. ***P < 0.001 control compared to serum stimulation, two-tailed homoscedastic Student’s t test. Data are means ± SEM; n = 3 biological replicates per group.