Table 1: Water contact angles on bovine dentin treated with different conditioning and peptide-coating protocols.
Columns from left to right; Etch: 32% phosphoric acid etched dentin; EtOH: 100% ethanol rinse; 1Coat: 1st peptide coating; 2Coat: 2nd peptide coating; H2O: water rinse; Prog.EtOH: progressive alcohol dehydration (50%, 70%, 80%, 95% and 100% ethanol); WCA±S.D.: final water contact angle ± standard deviation (n=7); Δ WCA: average variation of WCA over time (21s). Rows, from top to bottom; Cut Dentin: freshly cut coronal dentin; Etched Dentin: dentin etched with 32% phosphoric acid; Buffer-1coat- H2O: 1st buffer (Na2CO3) coating followed by water rinse; Buffer-1coat- EtOH: 1st buffer (Na2CO3) coating followed by 100% ethanol rinse; Buffer-2coats-2C-Prog.EtoH: 2nd buffer (Na2CO3) coating followed by progressive alcohol dehydration (50%, 70%, 80%, 95% and 100%); GL13K-1coat-EtOH (Single coating-EtOH): 1st GL13K coating followed by 100% ethanol rinse; GL13K-1coat-H2O (Single coating): 1st GL13K coating followed by water rinse; GL13K-2coat-H2O (Double coatings): double GL13K coating followed by water rinse; GL13K-2coats-EtOH Priming: double GL13K coating preceded by 100% ethanol priming; GL13K-2coats-1C-EtoH: double GL13K coatings separated by 100% ethanol rinse; GL13K-2coats-2C-EtoH (Double coatings-EtOH): double GL13K coatings followed by 100% ethanol rinse; GL13K-2coats-2C-PrEtoH (Double coatings-Prog.EtOH): double GL13K coatings followed by progressive alcohol dehydration (50%, 70%, 80%, 95% and 100%).
| Treatment | Pre-coating (conditioning) |
1st coating | 2nd coating | WCA±S.D. [°] |
Δ WCA [°] |
Image | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Etch | EtOH | 1coat | EtOH | 2coat | H2O | EtOH | Prog.EtOH | ||||
| Cut Dentin | 60±5 | 6 |  |
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| Etched Dentin | X | 20±9 | 25 |  |
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| Buffer-1coat- H2O | X | X | X | 45±11 | 20 |  |
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| Buffer-1coat- EtOH | X | X | X | 45±10 | 5 |  |
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| Buffer-2coats-2C-Prog.EtoH | X | X | X | X | 60±12 | 18 |  |
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| GL13K-1coat-EtOH (Single coating-EtOH) | X | X | X | 55±9 | 5 |  |
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| GL13K-1coat-H2O (Single coating) | X | X | X | 75±12 | 15 |  |
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| GL13K-2coat-H2O (Double coatings) | X | X | X | X | 70±11 | 17 |  |
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| GL13K-2coats-EtOH Priming | X | X | X | X | 80±8 | 12 |  |
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| GL13K-2coats-1C-EtoH | X | X | X | X | 90±8 | 8 |  |
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| GL13K-2coats-2C-EtoH (Double coatings-EtOH) | X | X | X | X | 100±8 | 12 |  |
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| GL13K-2coats-2C-PrEtoH (Double coatings-Prog.EtOH) | X | X | X | X | 120±5 | 4 |  |
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