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. 2019 Apr 12;10:801. doi: 10.3389/fmicb.2019.00801

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Copyright © 2019 Robertson, McGoverin, Vanholsbeeck and Swift.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relationship between live/dead spectrometry and viable cell plate counts in minimal media and saline. SYTO 9 emissions in minimal media (A; circles), PI emissions in minimal media (B; triangles), the adjusted dye ratio in minimal media (C; diamonds), and the adjusted dye ratio in saline (D; diamonds) compared to viable cell plate counts (A–D; squares) for live and dead E. coli suspensions. Fluorescence intensity was obtained from integrating 505–515 nm and 600–610 nm. Data presented is the median with the range from six biological replicates.