Fig. 3.

FLNC silencing inhibits invasiveness in GBM cell lines. a FLNC mRNA and protein levels of control and U87MG (left) and KNS81 (right) FLNC knockdown (sh) cells, as determined by qRT-PCR and immunoblotting, respectively. GAPDH was used as a loading control. b Representative images from the Transwell migration and invasion assays of control and FLNC-depleted cells. Original magnification: ×200; scale bar: 500 μm. c Quantification of control and FLNC sh cell migration and invasion. I/M indicates the invasion/migration ratio. Columns represent total cell number in five independent microscopic fields and bars indicate SD. NS not significant; **P < 0.01. GBM glioblastoma multiforme, GAPDH glyceraldehyde 3-phosphate dehydrogenase