Fig. 4.

Between-group analysis (BGA) of the soil microbial communities. The figure shows constrained principal component analysis (PCA) of the microbiome profiles after applying sample grouping, according to replicates for frozen and thaw samples along recorded temperature. Non-random distribution of the BGA grouping was tested using a Monte–Carlo simulation with 10.000 permutations (p<1.0E-04***)