Table 2.
Primers used to detect dfr genes.
| Primer | Sequence (5′–3′) | Amplicon size (bp) | References | |
|---|---|---|---|---|
| dfrA1 | D1 | ACGGATCCTGGCTGTTGGTTGGAC | 257 | Lee et al., 2001 |
| D2 | GCAATTCACCTTCCGGCTCGATGTC | |||
| dfrA5∗ | D3 | GTTGCGGTCCAGACATAC | 253 | Lee et al., 2001 |
| dfrA14∗ | D4 | CCGCCACCAGACACTA | ||
| dfrA6 | DfrA6-F | ATATCTCTTATGGCAGCTGTTTCC | 420 | This study |
| DfrA6-R | ACTTTGCTCAAATGTTTTGCTTG | |||
| dfrA7 | DfrA7-F | TCTGCAACGTCAGAAAATGG | 404 | This study |
| DfrA7-R | TGCTCAAAAACCAAATTGAAA | |||
| dfrA8 | D5 | TCGAGCTTCATGCCATTT | 454 | Lee et al., 2001 |
| D6 | TCTTCCATGCCATTCTGC | |||
| dfrA9 | D11 | CAGATTCCGTGGCATGAACC | 390 | Toleman et al., 2007 |
| D12 | GACCTCAGATACGAGTTTCC | |||
| dfrA10 | DfrA10-F | TATCACTTATCTTTGCGA | 537 | Grape et al., 2005 |
| DfrA10-R | GGCACCCCAACCAGCGAA | |||
| dfrA12 | DfrA12-F | TTTATCTCGTTGCTGCGATG | 457 | This study |
| DfrA12-R | TAAACGGAGTGGGTGTACGG | |||
| dfrA13 | DfrA13-F | TCGGAAAAGAGGGAAAGATG | 401 | This study |
| DfrA13-R | GGAGGTCTCCCCTCCTACCT | |||
| dfrA17 | DfrA17-F | CCGCTTAATCGGTAGTGGTC | 432 | This study |
| DfrA17-R | TTTTTCCAAATCCGGAATGTAT | |||
| dfrA20 | D15 | GGGAAACACCGAGAAATGGG | 407 | Toleman et al., 2007 |
| D16 | TTCTTCTTCCCATTCTCCCC | |||
| dfrBl, dfrB2 | Dfr11-F | GATCACGTGCGCAAGAAATC | 141 | Navia et al., 2003 |
| dfrB3∗∗ | Dfr11-R | AAGCGCAGCCACAGGATAAAT |
∗Primers used to amplify dfrA5 gene also amplify the dfrA14 gene. ∗∗Primers used to amplify dfrB1 gene also amplify the dfrB2 and dfrB3 genes.